Scott Ensign

Publications | Books

An asterisk (*) at the end of a publication indicates that it has not been peer-reviewed.

Publications | Journal Articles

Academic Journal

• Ensign, S.A, (2011). A new pathway for acetate assimilation. Science, 331, 294-295.
• Pandey, A.S, Mulder, D.W, Ensign, S.A, Peters, J.W, (2011). Structural basis for carbon dioxide binding by 2-ketopropyl coenzyme M oxidoreductase/carboxylase. FEBS Letters, 585, 459-464.
• Kofoed, M.A, Wampler, D.A, Pandey, A.S, Peters, J.W, Ensign, S.A, (2011). Roles of the redox active disulfide, and histidine residues forming a catalytic dyad, in reactions catalyzed by 2-Ketopropyl-Coenzyme M Oxidoreductase/Carboxylase. The Journal of Bacteriology, 193, 4904-4913.
• Boyd, J.M, Clark, D.D, Kofoed, M.A, Ensign, S.A, (2010). Mechanism of inhibition of aliphatic epoxide carboxylation by the Coenzyme M analog 2-bromoethanesulfonate. Journal of Biological Chemistry, 285, 25232-25242.
• Sliwa, D., Krishnakumar, A., Peters, J.W, Ensign, S.A, (2010). Molecular basis for enantioselectivity in the (R)- and (S)-hydroxypropylthioethanesulfonate dehydrogenases, a unique pair of stereoselective short-chain dehydrogenases/reductases involved in aliphatic epoxide carboxylation. Biochemistry, 49, 8831-8840.
• Krishnakumar, A.M, Sliwa, D., Endrizzi, J.A, Boyd, E.S, Ensign, S.A, Peters, J.W, (2008). Getting a Handle on the Role of Coenzyme M in Alkene Metabolism. Microbiology and Molecular Biology Reviews, 72, 445-456.
• Boyd, J.M, Ellsworth, A., Ensign, S.A, (2006). Characterization of 2-Bromoethanesulfonate as a Selective Inhibitor of the Coenzyme M-Dependent Pathway and Enzymes of Bacterial Aliphatic Epoxide Metabolism. The Journal of Bacteriology, 188, 8062-8069.
• Pandev, A.S, Nocek, B., Clark, D.D, Ensign, S.A, Peters, J.W, (2006). Mechanistic Implications of the Structure of the Mixed-Disulfide Intermediate of the Disulfide Oxidoreductase, 2-Ketopropyl-Coenzyme M Oxidoreductase/Carboxylase. Biochemistry, 45, 113-120.
• Ensign, S.A, (2006). Revisiting the glyoxylate cycle: alternative pathways for microbial acetate assimilation. Molecular Microbiology, 61, 274-276.
• Krishnakumar, A.M, Nocek, B.P, Clark, D.D, Ensign, S.A, Peters, J.W, (2006). Structural Basis for Stereoselectivity in the (R)- and (S)-Hydroxypropylthioethanesulfonate Dehydrogenases. Biochemistry, 45, 8831-8840.
• Boyd, J.M, Ensign, S.A, (2005). ATP-dependent Enolization of Acetone by Acetone Carboxylase from Rhodobacter capsulatus. Biochemistry, 44, 8543-8553.
• Boyd, J.M, Ensign, S.A, (2005). Evidence for a Metal-Thiolate Intermediate in Alkyl Group Transfer from Epoxypropane to Coenzyme M and Cooperative Metal Ion Binding in Epoxyalkane:CoM Transferase. Biochemistry, 44, 13151-13162.
• Wampler, D.A, Ensign, S.A, (2005). Photoheterotrophic metabolism of Acrylamide by a Newly Isolated Strain of Rhodopseudomonas palustris. Appl. Environ. Microbiol., 71, 5850-5857.
• Boyd, J.M, Ellsworth, H., Ensign, S.A, (2004). Bacterial acetone carboxylase is a manganese-dependent metalloenzyme. The Journal of Biological Chemistry, 279, 46644-46651.
• Nocek, B., Boyd, J., Ensign, S.A, Peters, J.W, (2004). Crystallization and preliminary X-ray analysis of an acetone carboxylase from Xanthobacter autotrophicus strain Py2. ACTA CRYSTALLOGRAPHICA D, 60, 385-387.
• Clark, D.D, Boyd, J.M, Ensign, S.A, (2004). The Stereoselectivity and Catalytic Properties of Xanthobacter autotrophicus 2-[(R)-2-Hydroxypropylthio]ethanesulfonate Dehydrogenase Are Controlled by Interactions between C-Terminal Arginine Residues and the Sulfonate of Coenzyme M. Biochemistry, 43, 6763-6771.
• Ensign, S.A, Allen, J.R, (2003). Aliphatic Epoxide Carboxylation. Annual Review of Biochemistry, 72, 55-76.
• Clark, D.D, Ensign, S.A, (2002). Characterization of the 2-[(R)-2-hydroxypropylthio] ethanesulfonate Dehydrogenase from Xanthobacter strain Py2: Product Inhibition, pH-Dependence of Kinetic Parameters, Site-Directed Mutagenesis, Rapid-Equilibrium Inhibition, and Chemical Modification. Biochemistry, 41, 2727-2740.
• Nocek, B., Clark, D.D, Ensign, S.A, Peters, J.W, (2002). Crystallization and preliminary X-ray analysis of an R-2-hydroxypropyl-coenzyme M dehydrogenase. Acta Crystallographica, 58, 1470-1473.
• Nocek, B., Jang, S.B, Jeong, M.S, Clark, D.D, Ensign, S.A, Peters, J.W, (2002). Structural basis for CO2 fixation by a novel member of the disulfide oxidoreductase family of enzymes: 2-ketopropyl-Coenzyme M Oxidoreductase/ Carboxylase. Biochemistry, 41, 12907-12913.
• Sluis, M.K, Larsen, R.A, Krum, J.G, Anderson, R., Metcalf, W.W, Ensign, S.A, (2002). Biochemical, molecular, and genetic analyses of the acetone carboxylases from Xanthobacter autotrophicus strain Py2 and Rhodobacter capsulatus strain B10. J Bacteriol. , 184:11, 2969-2977.
• Krum, J.G, Ellsworth, H., Sargeant, R.R, Rich, G., Ensign, S.A, (2002). Kinetic and microcalorimetric analysis of substrate and cofactor interactions in epoxyalkane:CoM transferase, a zinc-dependent epoxidase. Biochemistry, 15, 5005-5014.
• Jang, S.B, Jeong, M.S, Clark, D.D, Ensign, S.A, Peters, J.W, (2001). Characterization and preliminary X-ray analysis of a NADPH:2-ketopropyl-Coenzyme M Oxidoreductase/ Carboxylase. Acta Crystallographica, 57, 445-447.
• Krum, J.G, Ensign, S.A, (2001). Evidence that a linear megaplasmid in Xanthobacter strain Py2 encodes enzymes of aliphatic alkene and epoxide metabolism and Coenzyme M (2-mercaptoethanesulfonate) biosynthesis. J. Bacteriol. , 183, 2172-2177.
• Ensign, S.A, (2001). Microbial Metabolism of Aliphatic Alkenes. Biochemistry, 40, 5845-5853.

An asterisk (*) at the end of a publication indicates that it has not been peer-reviewed.

Publications | Other

An asterisk (*) at the end of a publication indicates that it has not been peer-reviewed.